People are encouraged to attend the two training sessions in a short time frame between each other. Training consists of two sessions in which modalities of sorting and instrument set ups are illustrated (see instructions and training section).
Trained operators will be able to reserve time directly on the AriaII-2 schedule. High-speed cell sorting (self-operated, AriaII-2) changes in nozzle size, changes in pressure, single cell sorting into 96- or 384- well plates), please include this information in the notes section of the sorting request form and add 30 minutes to your requested sorting time to allow changes from the default setup (NOTE: you will be charged for this special setup time). If your sort requires a special setup (i.e. If the setup notes for your sorting do not indicate special requirements, the AriaII-1 will be set up with a default 85um nozzle 45psi. If you do not receive a confirmation email, your sort has not been scheduled. You will receive a confirmation email when your request has been added to the schedule. An email will be sent to the FACS staff detailing your request and your request will be added to the sorting calendar. To schedule cell sorting on the Aria II-1, go to the online schedule, choose the time you would like to begin and end, click on REQUEST and fill out the pop-up window with your lab and sort information.
Requests for cell sorting can be made using the online schedule or by contacting the FACS Staff by email at The online schedule is accessible only to Washington University researchers and requires a username and password. High-speed cell sorting (staff-operated, AriaII-1)Īppointments with staff are available Monday through Friday from 10am to 5pm. Please note that each user needs to have an individual account. First time users: please register here. When you log in fill in all required information and request access to instruments. Please allow one business day for the activation of your account. Contact the FACS Staff if you have any questions or problems with the new account at and pricing Service available to: All entities, including for-profit organizations. Consultations on sample preparation and data analysis.Instruction and training on the instruments.The Flow Cytometry Core provides investigators with instrumentation and support for cell sorting as well as acquisition and analysis of flow cytometry data. Physician Scientist Training Program Directory.Faculty Development Faculty Development.Barnes-Jewish Hospital Molecular Diagnostics Laboratory.Clinical Laboratory Sections and Medical Directorships.Cytogenetics and Molecular Pathology (CMP) Laboratory.patents that may apply, see bd.com/patents. Please refer to to access safety data sheets (SDS).We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at.Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions.Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.An isotype control should be used at the same concentration as the antibody of interest.
We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
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